pDONR223-MST1R
(Plasmid #23942)

• Depositing Labs: William Hahn, David Root
• Publication: Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627

Backbone

• Vector backbone: pDONR223
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5005
• Vector type: Gateway Donor vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MST1R
• Alt name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• Species: H. sapiens (human)
• Insert Size (bp): 4203
• Mutation: R322Q
• Entrez Gene: MST1R (a.k.a. CD136, CDw136, NPCA3, PTK8, RON, SEA, p185-Ron)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The ORF has a Stop codon.

A set of 4 custom lentiviral vectors is available (pLX301 http://www.addgene.org/25895 , pLX302 http://www.addgene.org/25896 , pLX303 http://www.addgene.org/25897 , pLX304 http://www.addgene.org/25890 ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Addgene QC NGS analysis identified a R322Q discrepancy relative to NCBI reference sequence NP_002438.2. It is unknown if this may impact plasmid function.