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HDM_RSV_B_OR795252_F_N201S
(Plasmid #237363)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HDM
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RSV B F human codon optimized from Genbank Accesion OR79525
  • Species
    RSV
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clinical sequence selected for presence of N201S in RSV F, a known nirsevimab escape mutant.

Please visit https://doi.org/10.1101/2025.03.11.642476 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HDM_RSV_B_OR795252_F_N201S was a gift from Jesse Bloom (Addgene plasmid # 237363 ; http://n2t.net/addgene:237363 ; RRID:Addgene_237363)

  • For your References section:

    RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera. Simonich CAL, McMahon TE, Ju X, Yu TC, Brunette N, Stevens-Ayers T, Boeckh MJ, King NP, Greninger AL, Bloom JD. bioRxiv 2025.03.11.642476; doi: 10.1101/2025.03.11.642476 10.1101/2025.03.11.642476
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