HDM_RSV_B1_F
(Plasmid
#237353)
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PurposeExpression plasmid for RSV B1 F
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneHDM
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRSV B1 F human codon optimized from Genbank Accesion AF013254
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SpeciesRSV
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Strain also referred to as RSV BWV40167-85.
Please visit https://doi.org/10.1101/2025.03.11.642476 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HDM_RSV_B1_F was a gift from Jesse Bloom (Addgene plasmid # 237353 ; http://n2t.net/addgene:237353 ; RRID:Addgene_237353) -
For your References section:
RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera. Simonich CAL, McMahon TE, Ju X, Yu TC, Brunette N, Stevens-Ayers T, Boeckh MJ, King NP, Greninger AL, Bloom JD. bioRxiv 2025.03.11.642476; doi: 10.1101/2025.03.11.642476 10.1101/2025.03.11.642476