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Addgene

Ec dnaN-pHK/P
(Plasmid #237239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-16b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dnaN (beta), N-terminal his-kinase cleavable tag
  • Species
    E. coli
  • Entrez Gene
    dnaN (a.k.a. b3701, ECK3693)
  • Tag / Fusion Protein
    • his-10+PKA kinase motif+PreScission Protease cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The PKA kinase site and PreScission Protesase clevage site are added between the his-10 tag & Factor Xa sequence in pET(16b) and the start of the Ec dnaN ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ec dnaN-pHK/P was a gift from Michael O'Donnell (Addgene plasmid # 237239 ; http://n2t.net/addgene:237239 ; RRID:Addgene_237239)
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