Ec holB-pET(11a)(ACH-)
(Plasmid
#237234)
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Purposeover-express E. coli holB (Pol III delta prime subunit)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-11a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameholB (delta prime)
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SpeciesE. coli
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Entrez GeneholB (a.k.a. b1099, ECK1085)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Aat II, Cla I, & Hind III sites eliminated from plasmid backbone
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ec holB-pET(11a)(ACH-) was a gift from Michael O'Donnell (Addgene plasmid # 237234 ; http://n2t.net/addgene:237234 ; RRID:Addgene_237234)