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Addgene

pAG GST EGFP
(Plasmid #236736)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236736 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pAG
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 4944
  • Total vector size (bp) 5661
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Synthetic
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cggctcgtataatgtgtgga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG GST EGFP was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 236736 ; http://n2t.net/addgene:236736 ; RRID:Addgene_236736)
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