DONOR plasmid
(Plasmid
#236552)
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PurposeContains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloTag
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
the IRES sequence has a single base-pair deletion (C) at position 20
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DONOR plasmid was a gift from Pablo Rivera-Fuentes (Addgene plasmid # 236552 ; http://n2t.net/addgene:236552 ; RRID:Addgene_236552)