cACTB
(Plasmid
#236544)
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PurposeFor expression of ACTB in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 236544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRE2-hyg
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACTB
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SpeciesCanis lupus familiaris (dog)
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Insert Size (bp)1125
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MutationWT
- Promoter hpACTB
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S2926 (Plasmid collection number)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cACTB was a gift from Sandra Citi (Addgene plasmid # 236544 ; http://n2t.net/addgene:236544 ; RRID:Addgene_236544) -
For your References section:
A feedback circuitry involving gamma-actin, beta-actin and nonmuscle myosin-2 A controls tight junction and apical cortex mechanics. Mauperin M, Sun Y, Glandorf T, Oswald TA, Klatt N, Geil B, Mutero-Maeda A, Mean I, Jond L, Janshoff A, Yan J, Citi S. Nat Commun. 2025 Mar 13;16(1):2514. doi: 10.1038/s41467-025-57428-y. 10.1038/s41467-025-57428-y PubMed 40082413