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Addgene

cACTG1
(Plasmid #236543)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236543 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRE2-hyg
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACTG1
  • Species
    Canis lupus familiaris (dog)
  • Insert Size (bp)
    1154
  • Mutation
    WT
  • Promoter hpACTB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S2882 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cACTG1 was a gift from Sandra Citi (Addgene plasmid # 236543 ; http://n2t.net/addgene:236543 ; RRID:Addgene_236543)

  • For your References section:

    A feedback circuitry involving gamma-actin, beta-actin and nonmuscle myosin-2 A controls tight junction and apical cortex mechanics. Mauperin M, Sun Y, Glandorf T, Oswald TA, Klatt N, Geil B, Mutero-Maeda A, Mean I, Jond L, Janshoff A, Yan J, Citi S. Nat Commun. 2025 Mar 13;16(1):2514. doi: 10.1038/s41467-025-57428-y. 10.1038/s41467-025-57428-y PubMed 40082413
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