hPDZD11-HA
(Plasmid
#236537)
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PurposeFor expression of PDZD11 (human) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236537 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePDZD11
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SpeciesH. sapiens (human)
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Insert Size (bp)420
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MutationWT
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Entrez GenePDZD11 (a.k.a. AIPP1, PDZK11, PISP)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (unknown if destroyed)
- 3′ cloning site Xba1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S1766 (Plasmid collection number)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hPDZD11-HA was a gift from Sandra Citi (Addgene plasmid # 236537 ; http://n2t.net/addgene:236537 ; RRID:Addgene_236537) -
For your References section:
PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins. Guerrera D, Shah J, Vasileva E, Sluysmans S, Mean I, Jond L, Poser I, Mann M, Hyman AA, Citi S. J Biol Chem. 2016 May 20;291(21):11016-29. doi: 10.1074/jbc.M115.712935. Epub 2016 Apr 4. 10.1074/jbc.M115.712935 PubMed 27044745