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Addgene

HA-hNM2B
(Plasmid #236533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-Zeo+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MYH10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5928
  • Mutation
    WT
  • Entrez Gene
    MYH10 (a.k.a. NMMHC-IIB, NMMHCB)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S2749 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-hNM2B was a gift from Sandra Citi (Addgene plasmid # 236533 ; http://n2t.net/addgene:236533 ; RRID:Addgene_236533)

  • For your References section:

    Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane. Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S, Mutero-Maeda A, Yan J, Citi S. J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 10.1083/jcb.202208065. Epub 2023 May 19. 10.1083/jcb.202208065 PubMed 37204781
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