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YFP-cCGNL1-cCGN_D-Myc
(Plasmid #236525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRE2-hyg
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CGNL1-CGN
  • Species
    Canis lupus familiaris (dog)
  • Insert Size (bp)
    3576
  • Mutation
    WT
  • Entrez Gene
    CGN
  • Promoter CMV
  • Tags / Fusion Proteins
    • YFP (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S1606 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-cCGNL1-cCGN_D-Myc was a gift from Sandra Citi (Addgene plasmid # 236525 ; http://n2t.net/addgene:236525 ; RRID:Addgene_236525)

  • For your References section:

    Distinct domains of paracingulin are involved in its targeting to the actin cytoskeleton and regulation of apical junction assembly. Paschoud S, Guillemot L, Citi S. J Biol Chem. 2012 Apr 13;287(16):13159-69. doi: 10.1074/jbc.M111.315622. Epub 2012 Feb 7. 10.1074/jbc.M111.315622 PubMed 22315225
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