cCGNL1-HA
(Plasmid
#236518)
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PurposeFor expression of cParacingulin L1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1A+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCGNL1
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SpeciesCanis lupus familiaris (dog)
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Insert Size (bp)3885
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MutationWT
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Entrez GeneCGNL1
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site Not1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S2432 (Plasmid collection number)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cCGNL1-HA was a gift from Sandra Citi (Addgene plasmid # 236518 ; http://n2t.net/addgene:236518 ; RRID:Addgene_236518) -
For your References section:
Paracingulin recruits CAMSAP3 to tight junctions and regulates microtubule and polarized epithelial cell organization. Flinois A, Mean I, Mutero-Maeda A, Guillemot L, Citi S. J Cell Sci. 2024 Mar 1;137(5):jcs260745. doi: 10.1242/jcs.260745. Epub 2023 May 15. 10.1242/jcs.260745 PubMed 37013686