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Addgene

YFP-cCGNL1-Myc
(Plasmid #236513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRE2-hyg
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CGNL1
  • Species
    Canis lupus familiaris (dog)
  • Insert Size (bp)
    4665
  • Mutation
    WT
  • Entrez Gene
    CGNL1
  • Tags / Fusion Proteins
    • YFP (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S1137 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-cCGNL1-Myc was a gift from Sandra Citi (Addgene plasmid # 236513 ; http://n2t.net/addgene:236513 ; RRID:Addgene_236513)

  • For your References section:

    Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression. Paschoud S, Citi S. Mol Membr Biol. 2008 Jan;25(1):1-13. doi: 10.1080/09687680701474009. 10.1080/09687680701474009 PubMed 18097951
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