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Addgene

GFP-hCGN
(Plasmid #236507)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236507 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1A
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CGN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3609
  • Mutation
    WT
  • Entrez Gene
    CGN
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (unknown if destroyed)
  • 3′ cloning site Acc651 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S2508 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-hCGN was a gift from Sandra Citi (Addgene plasmid # 236507 ; http://n2t.net/addgene:236507 ; RRID:Addgene_236507)

  • For your References section:

    Cingulin binds to the ZU5 domain of scaffolding protein ZO-1 to promote its extended conformation, stabilization, and tight junction accumulation. Vasileva E, Spadaro D, Rouaud F, King JM, Flinois A, Shah J, Sluysmans S, Mean I, Jond L, Turner JR, Citi S. J Biol Chem. 2022 Apr;298(4):101797. doi: 10.1016/j.jbc.2022.101797. Epub 2022 Mar 5. 10.1016/j.jbc.2022.101797 PubMed 35259394
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