xenCGN(1-378)
(Plasmid
#236504)
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PurposeFor expression of the head domain of Xenopus CGN in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236504 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCGN
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SpeciesX. laevis (frog)
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Insert Size (bp)1100
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MutationWT
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Entrez Genecgn (a.k.a. MGC98860)
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Tags
/ Fusion Proteins
- His (N terminal on insert)
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (unknown if destroyed)
- 3′ cloning site Kpu1 (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S5 (Plasmid collection number)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
xenCGN(1-378) was a gift from Sandra Citi (Addgene plasmid # 236504 ; http://n2t.net/addgene:236504 ; RRID:Addgene_236504) -
For your References section:
Evidence for a functional interaction between cingulin and ZO-1 in cultured cells. D'Atri F, Nadalutti F, Citi S. J Biol Chem. 2002 Aug 2;277(31):27757-64. doi: 10.1074/jbc.M203717200. Epub 2002 May 21. 10.1074/jbc.M203717200 PubMed 12023291