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Addgene

hCGN(1-406)
(Plasmid #236503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CGN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Mutation
    WT
  • Entrez Gene
    CGN
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S562 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCGN(1-406) was a gift from Sandra Citi (Addgene plasmid # 236503 ; http://n2t.net/addgene:236503 ; RRID:Addgene_236503)

  • For your References section:

    Evidence for a functional interaction between cingulin and ZO-1 in cultured cells. D'Atri F, Nadalutti F, Citi S. J Biol Chem. 2002 Aug 2;277(31):27757-64. doi: 10.1074/jbc.M203717200. Epub 2002 May 21. 10.1074/jbc.M203717200 PubMed 12023291
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