pAPInt-mCherry-Nat
(Plasmid
#236488)
-
PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 236488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFA6A
-
Vector typeYeast Expression
-
Selectable markersNourseothricin (Nat)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemCherry
-
SpeciesAureobasidium pullulans
-
MutationCodon optimized for A. pullulans
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAPInt-mCherry-Nat was a gift from Daniel Lew (Addgene plasmid # 236488 ; http://n2t.net/addgene:236488 ; RRID:Addgene_236488) -
For your References section:
Optimized vectors for genetic engineering of Aureobasidium pullulans. Colarusso A, Williams A, Gladfelter AS, Wirshing ACE, Lew DJ. bioRxiv [Preprint]. 2025 Jan 27:2025.01.25.634885. doi: 10.1101/2025.01.25.634885. 10.1101/2025.01.25.634885 PubMed 39975359