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Addgene

pAP-U2-2
(Plasmid #236473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK (+)
  • Backbone manufacturer
    Stratagene
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spH2Bp-3xmCherry scACT1p-3xGFP
  • Species
    Aureobasidium pullulans
  • Mutation
    Codon optimized for A. pullulans

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAP-U2-2 was a gift from Daniel Lew (Addgene plasmid # 236473 ; http://n2t.net/addgene:236473 ; RRID:Addgene_236473)

  • For your References section:

    Optimized vectors for genetic engineering of Aureobasidium pullulans. Colarusso A, Williams A, Gladfelter AS, Wirshing ACE, Lew DJ. bioRxiv [Preprint]. 2025 Jan 27:2025.01.25.634885. doi: 10.1101/2025.01.25.634885. 10.1101/2025.01.25.634885 PubMed 39975359
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