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Addgene

pQCXIP-EGFP-APEX2-V5-EEA1
(Plasmid #236403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIP
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP-APEX2-V5-EEA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5757
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • APEX2 (N terminal on insert)
    • V5 (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    EEA1 cloned from Addgene #42307

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP-EGFP-APEX2-V5-EEA1 was a gift from Jared Rutter (Addgene plasmid # 236403 ; http://n2t.net/addgene:236403 ; RRID:Addgene_236403)

  • For your References section:

    A dual-purification system to isolate mitochondrial subpopulations. Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J. J Cell Sci. 2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693. 10.1242/jcs.263693 PubMed 40079232
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