pTriEx-SAMBA
(Plasmid
#236390)
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PurposeA new chemically induced proximity (CIP) tool using salicylic acid or aspirin after hydrolysis as the small molecule trigger
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTriEx
- Backbone size w/o insert (bp) 5745
- Total vector size (bp) 6381
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEvolved from NtNPR1 GENE
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Alt nameSAMBA
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SpeciesSynthetic
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Insert Size (bp)636
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XHOI (not destroyed)
- 5′ sequencing primer CCC CGT AAT GCA GAA GAA GA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTriEx-SAMBA was a gift from Yubin Zhou (Addgene plasmid # 236390 ; http://n2t.net/addgene:236390 ; RRID:Addgene_236390) -
For your References section:
Repurposing salicylic acid as a versatile inducer of proximity. Wang T, Liu S, Ke Y, Ali S, Wang R, Hong T, Liu Z, Ma G, Lan TH, Wang F, Zhu MX, Huang Y, Zhou Y. Nat Chem Biol. 2025 Jun 13. doi: 10.1038/s41589-025-01918-z. 10.1038/s41589-025-01918-z PubMed 40514548