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pLenti Puro HA-Ubiquitin-K48
(Plasmid #236290)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236290 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 7055
  • Total vector size (bp) 7283
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin K48
  • Alt name
    UBB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    228
  • Mutation
    Single ubiquitin coding sequence containing K48 mutation (full length UBB has three repeating ubiquitin coding sequences)
  • Entrez Gene
    UBB (a.k.a. HEL-S-50)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti Puro HA-Ubiquitin-K48 was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 236290 ; http://n2t.net/addgene:236290 ; RRID:Addgene_236290)
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