pAAV-hSyn-roxSTOProx-NaChBac-TdTomato
(Plasmid
#236253)
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PurposeDre dependent NaChBac-TdTomato expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 7777
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsGrow for minimum time necessary. Do not store colonies on plate.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNaChBac-TdTomato
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Insert Size (bp)2336
- Promoter hSyn
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Tag
/ Fusion Protein
- TdTomato
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byChristin Kosse, Hyun Ah Yi, and Jeffrey Friedman
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-roxSTOProx-NaChBac-TdTomato was a gift from Janelia Viral Tools (Addgene plasmid # 236253 ; http://n2t.net/addgene:236253 ; RRID:Addgene_236253) -
For your References section:
A subcortical feeding circuit linking an interoceptive node to jaw movement. Kosse C, Ivanov J, Knight Z, Pellegrino K, Friedman J. Nature. 2024 Dec;636(8041):151-161. doi: 10.1038/s41586-024-08098-1. Epub 2024 Oct 23. 10.1038/s41586-024-08098-1 PubMed 39443799