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Addgene

7AA-aSyn-pRK172
(Plasmid #236197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK172
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SNCA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    444
  • Mutation
    insertion os MAAAEKT at residue 22
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CTGAGAAAACCATGGCAGCAGCAGAAAAGACTAAACAGGGTGTG
  • 3′ sequencing primer CACACCCTGTTTGGCAGCAGCAGAAAAGACTATGGTTTTCTCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7AA-aSyn-pRK172 was a gift from Sjors Scheres & Yang Yang (Addgene plasmid # 236197 ; http://n2t.net/addgene:236197 ; RRID:Addgene_236197)

  • For your References section:

    New SNCA mutation and structures of alpha-synuclein filaments from juvenile-onset synucleinopathy. Yang Y, Garringer HJ, Shi Y, Lovestam S, Peak-Chew S, Zhang X, Kotecha A, Bacioglu M, Koto A, Takao M, Spillantini MG, Ghetti B, Vidal R, Murzin AG, Scheres SHW, Goedert M. Acta Neuropathol. 2023 May;145(5):561-572. doi: 10.1007/s00401-023-02550-8. Epub 2023 Feb 27. 10.1007/s00401-023-02550-8 PubMed 36847833
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