EW490 CMV-TO-UTRN-14ZF-realdeImmLink-NZF (FLP-IN)
(Plasmid
#236149)
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PurposePlasmid encoding the UTRN-14 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFLP-IN
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUTRN-14ZF-deImmLink-NZF
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SpeciesSynthetic
- Promoter CMV promoter with two TetR binding sites
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See https://benchling.com/s/seq-smC9jf2TiVg8kpSW9ITb?m=slm-DANTMVUzlaEOhpRvZkg0for annotated sequence. Please visit https://doi.org/10.1101/2025.02.24.639918 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EW490 CMV-TO-UTRN-14ZF-realdeImmLink-NZF (FLP-IN) was a gift from Xiaojing Gao (Addgene plasmid # 236149 ; http://n2t.net/addgene:236149 ; RRID:Addgene_236149) -
For your References section:
Machine-Guided Dual-Objective Protein Engineering for Deimmunization and Therapeutic Functions. Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh AA, Gao XJ. bioRxiv 2025.02.24.639918 10.1101/2025.02.24.639918