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Addgene

pAG Lenti CMV EGFP-HA Puro
(Plasmid #236082)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236082 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pAG Lenti
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 7057
  • Total vector size (bp) 7812
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Alt name
    717
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstBI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG Lenti CMV EGFP-HA Puro was a gift from Melina Fan (Addgene plasmid # 236082 ; http://n2t.net/addgene:236082 ; RRID:Addgene_236082)
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