pPGK-SB13
(Plasmid
#236078)
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PurposeSB13 transposase, for use with Sleeping Beauty transponson plasmids
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT
- Total vector size (bp) 4663
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Vector typeSleeping beauty (SB) transposase plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSB13 transposase
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SpeciesAtlantic Salmon
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Insert Size (bp)1023
- Promoter PGK
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPGK-SB13 was a gift from Masashi Narita (Addgene plasmid # 236078 ; http://n2t.net/addgene:236078 ; RRID:Addgene_236078) -
For your References section:
Titration of RAS alters senescent state and influences tumour initiation. Chan ASL, Zhu H, Narita M, Cassidy LD, Young ARJ, Bermejo-Rodriguez C, Janowska AT, Chen HC, Gough S, Oshimori N, Zender L, Aitken SJ, Hoare M, Narita M. Nature. 2024 Sep;633(8030):678-685. doi: 10.1038/s41586-024-07797-z. Epub 2024 Aug 7. 10.1038/s41586-024-07797-z PubMed 39112713