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Addgene

pTR01F/hFGFR4-V5
(Plasmid #236017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236017 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TR01F
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human FGFR4
  • Species
    H. sapiens (human)
  • Entrez Gene
    FGFR4 (a.k.a. CD334, JTK2, TKF)
  • Promoter truncated CMV promoter
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The TR01F plasmid was obtained as a gift from Valeri Mossine (PMID: 24376882).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTR01F/hFGFR4-V5 was a gift from Pavel Krejčí (Addgene plasmid # 236017 ; http://n2t.net/addgene:236017 ; RRID:Addgene_236017)

  • For your References section:

    Specific inhibition of fibroblast growth factor receptor 1 signaling by a DNA aptamer. Zlinska V, Feketova Z, Czyrek A, Chudzian J, Zivkovic ML, Ursachi VC, Dudeja P, Fafilek B, Rynes J, Rico-Llanos G, Koudelka A, Roy T, Biadun M, Raskova V, Svozilova K, Stroblova M, Krzyscik M, Hristova K, Krowarsch D, Foldynova-Trantirkova S, Zakrzewska M, Trantirek L, Krejci P. Mol Ther Nucleic Acids. 2024 Nov 28;36(1):102405. doi: 10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11. 10.1016/j.omtn.2024.102405 PubMed 39759879
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