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Addgene

pYO1125
(Plasmid #235742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPTG
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p40 PX domain
  • Species
    H. sapiens (human)
  • Mutation
    aa 2-149 with Cys at 2
  • Entrez Gene
    NCF4 (a.k.a. CGD3, NCF, P40PHOX, SH3PXD4)
  • Tag / Fusion Protein
    • GST-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For other work using this plasmid, please see Ohashi and Tremel et al. 2020 PMID 32602837; Tremel et al. 2021 PMID 33692360; Mercer et al. 2021 PMID 34121209.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO1125 was a gift from Roger Williams (Addgene plasmid # 235742 ; http://n2t.net/addgene:235742 ; RRID:Addgene_235742)

  • For your References section:

    Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes. Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL. Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365. 10.1126/science.aac7365 PubMed 26450213
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