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Addgene

pYO1645
(Plasmid #235719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235719 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPTG
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p40phox PX domain
  • Species
    H. sapiens (human)
  • Mutation
    Cys at 2, C85S, aa 2-149
  • Entrez Gene
    NCF4 (a.k.a. CGD3, NCF, P40PHOX, SH3PXD4)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO1645 was a gift from Roger Williams (Addgene plasmid # 235719 ; http://n2t.net/addgene:235719 ; RRID:Addgene_235719)

  • For your References section:

    The G-Protein Rab5A Activates VPS34 Complex II, a Class III PI3K, by a Dual Regulatory Mechanism. Buckles TC, Ohashi Y, Tremel S, McLaughlin SH, Pardon E, Steyaert J, Gordon MT, Williams RL, Falke JJ. Biophys J. 2020 Dec 1;119(11):2205-2218. doi: 10.1016/j.bpj.2020.10.028. Epub 2020 Oct 31. 10.1016/j.bpj.2020.10.028 PubMed 33137306
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