pOP795
(Plasmid
#235700)
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PurposeProtein expression His-taged HsNRBF2_FL in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235700 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOPTH
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNRBF2
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SpeciesH. sapiens (human)
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Entrez GeneNRBF2 (a.k.a. COPR, COPR1, COPR2, NRBF-2)
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Tag
/ Fusion Protein
- His6(TEV) (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOP795 was a gift from Roger Williams (Addgene plasmid # 235700 ; http://n2t.net/addgene:235700 ; RRID:Addgene_235700) -
For your References section:
Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex. Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O, Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi M, Ktistakis NT, Sachse C, Williams RL. Autophagy. 2016 Nov;12(11):2129-2144. doi: 10.1080/15548627.2016.1226736. Epub 2016 Sep 14. 10.1080/15548627.2016.1226736 PubMed 27630019