pGL3-Cyr61-rDMR
(Plasmid
#235692)
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PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Cyr61 with luciferase assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3-Promoter
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCyr61
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Alt nameCcn1
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SpeciesM. musculus (mouse)
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Entrez GeneCcn1 (a.k.a. Cyr61, Igfbp10)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-Cyr61-rDMR was a gift from Duojia Pan (Addgene plasmid # 235692 ; http://n2t.net/addgene:235692 ; RRID:Addgene_235692) -
For your References section:
YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesis. Wu BK, Mei SC, Chen EH, Zheng Y, Pan D. Nat Genet. 2022 Aug;54(8):1202-1213. doi: 10.1038/s41588-022-01119-7. Epub 2022 Jul 14. 10.1038/s41588-022-01119-7 PubMed 35835915