pCDH-CMV-V5-RFP-LATS1
(Plasmid
#235689)
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PurposeExpress RFP tagged LATS1 gene in mammalian cells using the CMV promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH-CMV-MCS-EF1-Puro
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLATS1
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SpeciesH. sapiens (human)
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Entrez GeneLATS1 (a.k.a. WARTS, wts)
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Tag
/ Fusion Protein
- V5-RFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-CMV-V5-RFP-LATS1 was a gift from Duojia Pan (Addgene plasmid # 235689 ; http://n2t.net/addgene:235689 ; RRID:Addgene_235689) -
For your References section:
Multiphase coalescence mediates Hippo pathway activation. Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H, Guo P, Chen EH, Zheng Y, Pan D. Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 10.1016/j.cell.2022.09.036. Epub 2022 Oct 31. 10.1016/j.cell.2022.09.036 PubMed 36318920