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pcDNA3.1-HA-RAD52-∆C
(Plasmid #235677)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235677 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)-N-HA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAD52
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    942
  • Mutation
    deleted amino acids 302-410
  • GenBank ID
    NM_134424.4
  • Entrez Gene
    RAD52
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer taatacgactcactataggg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-HA-RAD52-∆C was a gift from Simon Powell (Addgene plasmid # 235677 ; http://n2t.net/addgene:235677 ; RRID:Addgene_235677)

  • For your References section:

    RAD52 resolves transcription-replication conflicts to mitigate R-loop induced genome instability. Jalan M, Sharma A, Pei X, Weinhold N, Buechelmaier ES, Zhu Y, Ahmed-Seghir S, Ratnakumar A, Di Bona M, McDermott N, Gomez-Aguilar J, Anderson KS, Ng CKY, Selenica P, Bakhoum SF, Reis-Filho JS, Riaz N, Powell SN. Nat Commun. 2024 Sep 5;15(1):7776. doi: 10.1038/s41467-024-51784-x. 10.1038/s41467-024-51784-x PubMed 39237529
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