pcDNA3 JDM16-GFPnb-mCh
(Plasmid
#235667)
-
PurposeExpresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherry
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 235667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameJDM16-GFPnb-mCh
-
SpeciesSynthetic
-
Entrez GeneMch
- Promoter CMV
-
Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- GFP nanobody (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7 forward (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3 JDM16-GFPnb-mCh was a gift from David Baker (Addgene plasmid # 235667 ; http://n2t.net/addgene:235667 ; RRID:Addgene_235667) -
For your References section:
De novo designed Hsp70 activator dissolves intracellular condensates. Zhang JZ, Greenwood N, Hernandez J, Cuperus JT, Huang B, Ryder BD, Queitsch C, Gestwicki JE, Baker D. Cell Chem Biol. 2025 Feb 4:S2451-9456(25)00029-7. doi: 10.1016/j.chembiol.2025.01.006. 10.1016/j.chembiol.2025.01.006 PubMed 39922190