pCAGEN_V5_eMagAF_C74_cMycNLS
(Plasmid
#235640)
-
PurposeEncodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 235640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCAGEN
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameALIBi C-terminal fragment
-
Alt nameV5-eMagAF-C74-cMycNLS
-
SpeciesSynthetic
-
Mutationresidues 74-321
-
Tags
/ Fusion Proteins
- V5-eMagAF (N terminal on insert)
- cMycNLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAGEN_V5_eMagAF_C74_cMycNLS was a gift from Maria Barna (Addgene plasmid # 235640 ; http://n2t.net/addgene:235640 ; RRID:Addgene_235640) -
For your References section:
A subcellular map of translational machinery composition and regulation at the single-molecule level. Zhang Z, Xu A, Bai Y, Chen Y, Cates K, Kerr C, Bermudez A, Susanto TT, Wysong K, Garcia Marques FJ, Nolan GP, Pitteri S, Barna M. Science. 2025 Mar 7;387(6738):eadn2623. doi: 10.1126/science.adn2623. Epub 2025 Mar 7. 10.1126/science.adn2623 PubMed 40048539