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Addgene

pCAGEN_NES_V5_eMagAF_C196
(Plasmid #235625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGEN
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALIBi C-terminal fragment
  • Alt name
    NES-V5-eMagAF-C196
  • Species
    Synthetic
  • Mutation
    residues 196-321
  • Tag / Fusion Protein
    • NES-V5-eMagAF (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGEN_NES_V5_eMagAF_C196 was a gift from Maria Barna (Addgene plasmid # 235625 ; http://n2t.net/addgene:235625 ; RRID:Addgene_235625)

  • For your References section:

    A subcellular map of translational machinery composition and regulation at the single-molecule level. Zhang Z, Xu A, Bai Y, Chen Y, Cates K, Kerr C, Bermudez A, Susanto TT, Wysong K, Garcia Marques FJ, Nolan GP, Pitteri S, Barna M. Science. 2025 Mar 7;387(6738):eadn2623. doi: 10.1126/science.adn2623. Epub 2025 Mar 7. 10.1126/science.adn2623 PubMed 40048539
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