pCTcon2-dHUH
(Plasmid
#235187)
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PurposeTo display dHUH on yeast surface
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235187 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCTcon2
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedHUH
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Alt nameHUH-tag
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Alt namePCV2
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SpeciesPorcine circovirus type 2
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Insert Size (bp)345
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Tags
/ Fusion Proteins
- aga2p (N terminal on backbone)
- HA (N terminal on backbone)
- myc (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCTcon2-dHUH was a gift from Alice Ting (Addgene plasmid # 235187 ; http://n2t.net/addgene:235187 ; RRID:Addgene_235187) -
For your References section:
Directed evolution of a sequence-specific covalent protein tag for RNA labeling. Huang R, Ting AY. Proc Natl Acad Sci U S A. 2025 Mar 4;122(9):e2422085122. doi: 10.1073/pnas.2422085122. Epub 2025 Feb 26. 10.1073/pnas.2422085122 PubMed 40009639