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Addgene

pCTcon2-dHUH
(Plasmid #235187)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235187 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCTcon2
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dHUH
  • Alt name
    HUH-tag
  • Alt name
    PCV2
  • Species
    Porcine circovirus type 2
  • Insert Size (bp)
    345
  • Tags / Fusion Proteins
    • aga2p (N terminal on backbone)
    • HA (N terminal on backbone)
    • myc (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCTcon2-dHUH was a gift from Alice Ting (Addgene plasmid # 235187 ; http://n2t.net/addgene:235187 ; RRID:Addgene_235187)
  • For your References section:

    Directed evolution of a sequence-specific covalent protein tag for RNA labeling. Huang R, Ting AY. Proc Natl Acad Sci U S A. 2025 Mar 4;122(9):e2422085122. doi: 10.1073/pnas.2422085122. Epub 2025 Feb 26. 10.1073/pnas.2422085122 PubMed 40009639