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Addgene

pET23a-His-TEV-HaloTag-linker-SpyCatcher3
(Plasmid #235150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-23a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag linked to SpyCatcher3
  • Species
    Synthetic
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.28.560023 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-His-TEV-HaloTag-linker-SpyCatcher3 was a gift from Giordano Lippi (Addgene plasmid # 235150 ; http://n2t.net/addgene:235150 ; RRID:Addgene_235150)

  • For your References section:

    MicroRNA mechanisms instructing Purkinje cell specification. Zolboot N, Xiao Y, Du JX, Ghanem MM, Choi SY, Junn MJ, Zampa F, Huang Z, MacRae IJ, Lippi G. Neuron. 2025 Apr 1:S0896-6273(25)00181-3. doi: 10.1016/j.neuron.2025.03.009. 10.1016/j.neuron.2025.03.009 PubMed 40179877
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