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Addgene

pAAV-hSyn-FLAG-HA-T6B-MUT-YFP
(Plasmid #235140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mutant T6B peptide
  • Species
    H. sapiens (human)
  • Mutation
    5 tryptophan residues of T6B-WT mutated
  • Promoter hSyn
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SalI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.28.560023 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-FLAG-HA-T6B-MUT-YFP was a gift from Giordano Lippi (Addgene plasmid # 235140 ; http://n2t.net/addgene:235140 ; RRID:Addgene_235140)

  • For your References section:

    MicroRNA mechanisms instructing Purkinje cell specification. Zolboot N, Xiao Y, Du JX, Ghanem MM, Choi SY, Junn MJ, Zampa F, Huang Z, MacRae IJ, Lippi G. Neuron. 2025 Apr 1:S0896-6273(25)00181-3. doi: 10.1016/j.neuron.2025.03.009. 10.1016/j.neuron.2025.03.009 PubMed 40179877
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