OPEN-HLA-A*11:01-BirA
(Plasmid
#235135)
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PurposeE. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
- Backbone size w/o insert (bp) 3157
- Total vector size (bp) 3982
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOPEN-HLA-A*11:01
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SpeciesH. sapiens (human)
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MutationG120C
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Entrez GeneHLA-A (a.k.a. HLAA)
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Tag
/ Fusion Protein
- BirA (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OPEN-HLA-A*11:01-BirA was a gift from Nikolaos Sgourakis (Addgene plasmid # 235135 ; http://n2t.net/addgene:235135 ; RRID:Addgene_235135) -
For your References section:
Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. Sun Y, Young MC, Woodward CH, Danon JN, Truong HV, Gupta S, Winters TJ, Font-Burgada J, Burslem GM, Sgourakis NG. Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2304055120. doi: 10.1073/pnas.2304055120. Epub 2023 Jun 13. 10.1073/pnas.2304055120 PubMed 37310998