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pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry
(Plasmid #235096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-MCP-DEST-V5-mCherry
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry
  • Species
    H. sapiens (human)
  • Mutation
    The SRRM1 entry clone from DNASU was modified using a Q5 site directed mutagenesis kit (New England Biolabs) to delete the predicted region responsible for nuclear speckle localization as annotated using UniProt (https://www.uniprot.org/uniprotkb/Q8IYB3/entry)
  • Entrez Gene
    SRRM1 (a.k.a. 160-KD, POP101, SRM160)
  • Promoter CAG

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry was a gift from Mitchell Guttman (Addgene plasmid # 235096 ; http://n2t.net/addgene:235096 ; RRID:Addgene_235096)

  • For your References section:

    Genome organization around nuclear speckles drives mRNA splicing efficiency. Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh ST, Chari T, Sullivan DK, Blanco MR, Guttman M. Nature. 2024 May;629(8014):1165-1173. doi: 10.1038/s41586-024-07429-6. Epub 2024 May 8. 10.1038/s41586-024-07429-6 PubMed 38720076
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