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Addgene

pDest17_E3 ubiquitin-protein ligase (Topors) LCD (379-1045)
(Plasmid #235005)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235005 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest17
  • Backbone manufacturer
    GeneUniversal Inc.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E3 ubiquitin-protein ligase (Topors)
  • Species
    H. sapiens (human)
  • Entrez Gene
    TOPORS (a.k.a. LUN, P53BP3, RP31, TP53BPL)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Codon optimized gene synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TEV cleavage leaves N-terminal GS tag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest17_E3 ubiquitin-protein ligase (Topors) LCD (379-1045) was a gift from Tanja Mittag (Addgene plasmid # 235005 ; http://n2t.net/addgene:235005 ; RRID:Addgene_235005)
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