pDest17_HABP4 LCD (1-413)
(Plasmid
#235004)
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PurposeBacterial expression of N-terminally 6His tagged Intracellular HABP4 LCD
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest17
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Backbone manufacturerGeneUniversal Inc.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameIntracellular hyaluronan-binding protein 4
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SpeciesH. sapiens (human)
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Entrez GeneHABP4 (a.k.a. IHABP-4, IHABP4, Ki-1/57, SERBP1L)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byCodon optimized gene synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TEV cleavage leaves N-terminal GS tag
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDest17_HABP4 LCD (1-413) was a gift from Tanja Mittag (Addgene plasmid # 235004 ; http://n2t.net/addgene:235004 ; RRID:Addgene_235004)