Skip to main content
Addgene

pBS CMV NA 3C FLAG SA
(Plasmid #234993)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NA
  • Alt name
    Neuraminidase
  • Species
    Influenza A
  • Mutation
    In the Streptavidin coding sequence Glu44Val/Ser45Thr/Val47Arg
  • GenBank ID
    PV031350
  • Tag / Fusion Protein
    • HRV 3C site, FLAG, Strep-Tactin (SA) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains 5 silent mutations in Streptavidin. These silent mutations do not impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS CMV NA 3C FLAG SA was a gift from Monica Gonzalez-Magaldi & Daniel Leahy (Addgene plasmid # 234993 ; http://n2t.net/addgene:234993 ; RRID:Addgene_234993)

  • For your References section:

    Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography. Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AY, Guo L, Brilot A, Marcotte EM, Ke Z, Leahy DJ. bioRxiv [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 10.1101/2024.11.25.625301. 10.1101/2024.11.25.625301 PubMed 39651119
Related items: