pHA StreptagII 3C NIS EABR
(Plasmid
#234988)
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PurposeFor production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(-)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNIS-EABR
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Alt nameSodium-iodide symporter (NIS) fused to ESCRT and Alix binding region (EABR)
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SpeciesH. sapiens (human)
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GenBank IDPV031344
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Entrez GeneSLC5A5 (a.k.a. NIS, TDH1)
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Tag
/ Fusion Protein
- HA, Streptag-II, HRV 3C site (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHA StreptagII 3C NIS EABR was a gift from Monica Gonzalez-Magaldi & Daniel Leahy (Addgene plasmid # 234988 ; http://n2t.net/addgene:234988 ; RRID:Addgene_234988) -
For your References section:
Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography. Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AY, Guo L, Brilot A, Marcotte EM, Ke Z, Leahy DJ. bioRxiv [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 10.1101/2024.11.25.625301. 10.1101/2024.11.25.625301 PubMed 39651119