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Addgene

pEABR NA 3C FLAG SA
(Plasmid #234987)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234987 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EABR-NA
  • Alt name
    ESCRT and Alix binding region (EABR) fused to the transmembrane region of influenza neuraminidase (NA)
  • Species
    H. sapiens (human)
  • Mutation
    In the Streptavidin coding sequence Glu44Val/Ser45Thr/Val47Arg. Codon optimized for mammalian cell expression
  • GenBank ID
    PQ860840
  • Tag / Fusion Protein
    • HRV 3C site, FLAG, Strep-Tactin (SA) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains 5 silent mutations in Streptavidin. These silent mutations do not impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEABR NA 3C FLAG SA was a gift from Monica Gonzalez-Magaldi & Daniel Leahy (Addgene plasmid # 234987 ; http://n2t.net/addgene:234987 ; RRID:Addgene_234987)

  • For your References section:

    Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography. Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AY, Guo L, Brilot A, Marcotte EM, Ke Z, Leahy DJ. bioRxiv [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 10.1101/2024.11.25.625301. 10.1101/2024.11.25.625301 PubMed 39651119
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