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Addgene

pLVX-TetOne-Puro-UCP1-Flag
(Plasmid #234983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-TetOn-Puro
  • Backbone size w/o insert (bp) 9198
  • Total vector size (bp) 10176
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UCP1
  • Alt name
    Uncoupling Protein 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    978
  • GenBank ID
    NM_009463.3
  • Entrez Gene
    Ucp1 (a.k.a. Slc25a7, Ucp)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOne-Puro-UCP1-Flag was a gift from Denis Titov (Addgene plasmid # 234983 ; http://n2t.net/addgene:234983 ; RRID:Addgene_234983)

  • For your References section:

    Genetically encoded tool for manipulation of DeltaPsim identifies its role as the driver of ISR induced by ATP synthase dysfunction. Choe M, Ekvik AE, Stalnaker G, Shin HR, Titov DV. Cell Chem Biol. 2025 Apr 17;32(4):620-630.e6. doi: 10.1016/j.chembiol.2025.03.007. 10.1016/j.chembiol.2025.03.007 PubMed 40250406
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