pLVX-TRE3G-UCP1
(Plasmid
#234979)
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PurposeExpresses UCP1 in mouse cell lines
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234979 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-TRE3G
- Total vector size (bp) 8761
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUCP1
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Alt nameUncoupling Protein 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)924
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Entrez GeneUcp1 (a.k.a. Slc25a7, Ucp)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-TRE3G-UCP1 was a gift from Denis Titov (Addgene plasmid # 234979 ; http://n2t.net/addgene:234979 ; RRID:Addgene_234979) -
For your References section:
Genetically encoded tool for manipulation of DeltaPsim identifies its role as the driver of ISR induced by ATP synthase dysfunction. Choe M, Ekvik AE, Stalnaker G, Shin HR, Titov DV. Cell Chem Biol. 2025 Apr 17;32(4):620-630.e6. doi: 10.1016/j.chembiol.2025.03.007. 10.1016/j.chembiol.2025.03.007 PubMed 40250406