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Addgene

hSyn-IRES2-GFP
(Plasmid #234875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti SYN-FLAG-dCas9-VPR
  • Backbone manufacturer
    Jeremy Day (Addgene #114196)
  • Backbone size w/o insert (bp) 8573
  • Total vector size (bp) 9339
  • Modifications to backbone
    Inserted IRES2 site before EGFP
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    720
  • Promoter hSYn

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ctgcctcagtctgcggtg
  • 3′ sequencing primer cttgcttcctttggcgag
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Didier Trono (GFP amplified from Addgene #12252) and Pamela McLean (IRES site amplified from CIGW vector described in St. Martin et al., 2007; https://doi.org/10.1111/j.1471-4159.2006.04310.x)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn-IRES2-GFP was a gift from Andrew Arrant (Addgene plasmid # 234875 ; http://n2t.net/addgene:234875 ; RRID:Addgene_234875)

  • For your References section:

    Delivering progranulin to neuronal lysosomes protects against excitotoxicity. Davis SE, Roth JR, Aljabi Q, Hakim AR, Savell KE, Day JJ, Arrant AE. J Biol Chem. 2021 Sep;297(3):100993. doi: 10.1016/j.jbc.2021.100993. Epub 2021 Jul 21. 10.1016/j.jbc.2021.100993 PubMed 34298019
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